Semen cryopreservation is a major innovation in artificial insemination technology. It solved the problem long-term preservation of semen, so that semen from time, geography and animal life of the restrictions, greatly limits the fine to play and improve the utilization of stud ram, bred varieties and improved to accelerate the pace. Meanwhile, the good kind of ram for progeny test in the short term, retention and recovery of a species or individual ram fine selection of excellent properties, and in its descent update, introduction, and so reduce the production cost is important.
1, ejaculate
Fake vagina with semen collected by conventional methods. Semen collection frequency, generally continuous semen 2 days, rest one day. Day in the semen can be collected continuously 2) (interval 10-15 minutes). The vitality of the original semen requirements of not less than 0.8. The same operating procedures and routine artificial insemination.
Diluting
(A) Preparation of Frozen Semen
1, particle semen recipe
9-2 No. Liquid: I liquid, take 10 grams of lactose plus 80 ml of double distilled water, 20 ml of fresh skim milk, egg yolk 20 ml; II liquid; take I solution 45 ml, 3 g of glucose, glycerol 5 ml.
2, ampoules of semen recipe
Liquid Portuguese 3-3: I liquid, take glucose 3 g, 3 g sodium citrate, plus double distilled water to 100 ml, take 80 ml solution, add egg yolk 20 ml; II fluids, take I solution 44 ml, 6 ml of glycerol.
3, straw semen recipe
Portugal 3-3 to take liquid I liquid 94 ml, 6 ml of glycerol, plus 60 mg of selenium.
Of the above plus 100 ml of diluted penicillin 100 000 units, streptomycin 100 mg. Diluted solution used (except egg yolk) the need to filter and disinfect water-soluble in boiling, water vapor sterilization of glycerol alone. Dilution of the existing service is used.
(B) of the dilution ratio
Final dilution ratio (dilution ratio of semen in): particle semen 1:1, ampoules of semen and sperm for the 1:3 thin tube.
(C) the dilution method
Particles and ampoules of semen semen dilution by two, thin tube with a dilution of semen.
A dilution method: according to dilution ratio requirement, and a dilution-packing and sealing, wrapped in 8 layers of gauze decorated refrigerator temperature 3-4 degrees balance 3 hours.
Two dilution method: Using the non-glycerin solution I will be the proportion of sperm diluted to final concentration of 50%, 8 layers of gauze wrapped Later layout 3-4 degrees wide mouth bottle or jar of ice cooling balance (particles semen was 1-2 hours, safety bottles, straw semen to 2-3 hours), then with an equal amount of liquid solution containing glycerol group II second dilution.
Semen dilution as soon as possible after recovery, such as mixed-use multi-rams semen, or ejaculate too long, the need to adopt the semen of the first pre-diluted (before adding a small amount of diluted liquid) are each diluted should be under isothermal (semen and dilution isothermal) to complete.
Three-packing and frozen
(1)-packing seal
1, in small glass ampoules or plastic tube spinning number and frozen semen print date tag.
2, ampoules of semen, by the second diluted semen, immediately under-packing 3-4 degrees (1 ml ampoule) and flame sealed.
3, straw semen: with 5 ml syringe, at room temperature for 0.25 mm small-packing in the tube, and then with plastic beads or polyvinyl alcohol powder seal. Semen should not be too full of straw, sealing the middle after a certain gap should be retained to prevent the thawing of the small pipe burst.
(B) frozen
1, particle semen
The dry ice method: using a special bar hole, or ordinary glass rod in the pre-folded down, compacting the ice on the stamp at the neat round hole of light (diameter 0.4 cm, depth 2-2.5 cm) then mixing of semen dilution pipette by 0.1 ml capacity by freezing point drop, after 4-5 minutes picking, stored in liquid nitrogen. I am an expert from China Manufacturers, usually analyzes all kind of industries situation, such as skiving , threading machine.
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