High-speed centrifuge, centrifuge speed in the field of medical biology laboratories, hospitals, schools and universities, scientific research units for the separation of cells and bacteria; purification and separation of viruses, RNA, DNA, plasmid and protein bio- macromolecules.
HC Education Network High-speed Centrifuges , Speed centrifuge in the field of medical biology laboratories, hospitals, schools and universities, scientific research units for the separation of cells and bacteria; purification and separation of viruses, RNA, DNA, plasmids and proteins such as biological macromolecules. The following brief centrifuge operation, centrifugal process considerations, centrifugal Technology Common sense terms.
1 Basic Operation L) power is turned on. 2) select the time: according to "time" the selected time and press "enter,,.
3) Select Temperature: According to "temp" after the temperature selected by "enter".
4) Select the speed: Press "A m, 'the selected speed, then press" enter ".
5) Sample preparation: high-speed centrifuge tube volume increases the number of samples can be, but can not exceed the capacity of the pipe must be stamped with a balance (difference not too large) symmetric release the rotor. Most high-speed centrifuge rotor angle. Centrifuge tube capacity 50 mL of a 5 plus and so on. High speed centrifuge on different machines, and the requirements of high, it has a variety of rotor, angle type, horizontal, vertical, there are areas with the rotor, the sample must be filled to join, especially the level of the rotor. Seal affixed to the rotor angle is not subject to spills, balanced use and high accuracy Balance , Together with the casing (horizontal) and the lid and screw (rotor angle) with the balance. Symmetric placement and hanging. A 30mL centrifuge tube capacity of five such.
PC Tube: transparency, and hardness, can be heat sterilized, but the impatience of some organic solvents acid and alkali, mainly for speed 50,000 / min or more centrifugation. CN Tube: soft transparent texture, but the impatience of some organic solvents acid bases, do not heat sterilization, for sucrose, glycerol density gradient centrifugation, etc., because transparency is conducive to collect. lid sealing the tube is not good enough, the liquid can not fill (for high-speed centrifuge and use the angle of the rotor) to prevent spills, loss of balance. Spillover effects of the rotor and the centrifugal chamber of pollution affecting the sensors work properly. ultracentrifugation, the liquid must be filled tube, because the ultra-high vacuum from the need to pump, only to fill the tube to prevent deformation.
3 basic theory
Differential sedimentation centrifugation: differential sedimentation in a centrifuge tube of the suspension per injection, particles move to the tube during centrifugation the bottom, and in the bottom of the precipitation. Centrifugal timing is good, enough to make all of the largest particles are precipitated (Figure ZC), this would be no such particles in the supernatant, the supernatant can be centrifuged at higher speed to separate the second-largest particles and so on. Drawback is that one settlement will be uneven. I am China Manufacturers writer, reports some information about radiant hydronic heating , ptac units.
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