Seeds should maintain the absolute level of the element in order to germinate. Recombinant time super molecules are made during the endogenous expression of seed protein is suppressed, mounting levels of foreign protein boost believed to atone for ablation of endogenous protein synthesis. Thus, replacement of endogenous seed protein assembly in the foreign protein may be a promising approach to extend the performance of foreign recombinant protein. Provide an area for the deposition of recombinant super molecule in the daily range is important, as it can reduce any competition in the region between endogenous super molecules seed and introduced foreign protein. Installation profitability of many recombinant proteins is strongly supported by this strategy. The vaccinia virus / bacteriophage T7 expression system adapted to rodents ovary (CHO). Vaccinia virus infection is unsuccessful in CHO cells, which has a pointed reduce super molecule organic phase synthesis of intermediate infectious phenomena. We are trying to establish that expression of the T7 promoter-cistron acetyltransferase regulated antibiotic is at least twenty times more economical allowing BS-C-1, than in the CHO cells. Encephalitis virus 5'-untranslated region that confers cap independent translatability for RNA synthesis stimulated super molecule recombinant 10-fold in each cell line, while maintaining the advantage that the BS-C-1 cells than CHO cells. Since vaccinia virus cistron hour past poxvirus host restriction differ in CHO cells, we tend to make a recombinant virus that carried Associate in nursing intact cistron hour polymer in addition to the T7 enzyme cistron. With this virus, T7 polymer synthesis enzyme is increased and the production of recombinant super molecule in CHO cells, to the extent specified in the limit of the cell lines. Extending virus / bacteriophage expression system T7 vaccinia CHO cells should be great interest because these cells have a beneficial effect on the preparation of recombinant proteins in the analysis and biotechnology.
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